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Patent Number: |
5003050 |
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Case ID: |
0 |
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Patent Title:
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Diazoluminomelanin and a method for preparing same
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Status: |
ACTIVE |
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Status Date: |
6/3/2005 8:43:58 PM |
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Issue Date:
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3/26/1991 |
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Filed Date:
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3/7/1990 |
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Serial #:
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489561 |
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Assignee Name:
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The United States of America as represented by the Secretary of the Air (Washington, DC)
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Inventor(s):
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Kiel, Johnathan L. ,
O'Brien, Gerald J. |
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Lab Name: |
Human Effectiveness Directorate |
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Location: |
2610 Seventh Street, Building 441
Wright-Patterson AFB, OH 45433-7901 |
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Contact: |
Contact Lab About This Patent |
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BACKGROUND OF THE INVENTION
This invention relates to luminescent reagents.
Specific binding assays provide an economical means for detecting and
measuring an analyte present in low concentrations in a sample. Specific
binding assays are based upon the interaction of two bindable substances,
one the analyte and the other a specific binding partner, which
specifically recognize each other. Examples of specific binding partners
whose interaction can serve as the basis for a specific binding assay
include antigens-antibodies, biotin-avidin, nucleic acid probes,
enzymes-substrates, enzymes-inhibitors, enzymes-cofactors,
chelators-chelates, and cell surface receptor pairs. Assays involving
other specifically bindable substances are also known and within the scope
of the present invention. Specific binding assays have shown great utility
in determining various analytes in biological, medical, environmental,
agricultu . . . . More |
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A water-soluble luminescent compound having repeating units of the formula:
##STR1##
The product, which is a polymer having repeating units comprising
diazo-linked luminol and hydroxyindole, is referred to as
diazoluminomelanin (DALM), since one of the precursors to this product,
3-amino-L-tyrosine (3AT) is closely related to the biological substrates
which are converted into melanin.
Also provided is a method for preparing DALM which comprises reacting 3AT
with an alkali metal nitrite, and reacting the resulting diazonium salt
with luminol.
DALM can be used for chemiluminescent immunoassays for biological and
chemical agents; in radiofrequency and ionizing radiation dosimeters; and
for RNA/DNA hybridization assays for viruses and genetic detection.
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We claim:
1. A luminescent compound having repeating units of the formula:
##STR3##
2. A method for preparing a luminescent compound having repeating units of
the formula:
##STR4##
which comprises the steps of (a) reacting 3-amino-L-tyrosine with an
alkali metal nitrite to form a diazonium salt, (b) reacting said salt with
5-amino-2,3-dihydro-1,4-phthalazinedione and (c) recovering the resulting
compound.
3. The method of claim 2 wherein said reacting step (a) is carried out in
aqueous solution.
4. The method of claim 3 wherein said aqueous solution is acidic.
5. The method of claim 4 wherein said aqueous solution has a pH of about
5.0 to 6.0.
6. The method of claim 2 wherein said reacting step (a) is carried out for
about 1 to 20 minutes.
7. The method of claim 6 wherein said reacting step (a) is carried out for
about 5 to 10 minutes.
8. The method of claim 2 wherein . . . . More |
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